Composite

Part:BBa_K2557010:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-09)


TetO-miniCMV promoter-Bxb1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1099
    Illegal BamHI site found at 325
    Illegal BamHI site found at 540
    Illegal BamHI site found at 856
    Illegal BamHI site found at 1564
    Illegal BamHI site found at 1855
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 841
    Illegal AgeI site found at 331
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 561


Design Notes

SV40 NLS and flag was added by PCR.


Source

The TetO sequence was cloned from a Commercialized plasmid. The miniCMV promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the coding sequence of Bxb1 on NCBI and, after humanization, sent it to Kingsray for synthesis.

References